Predictika · Bioprocess development equipment

An AI sales agent's DASbox bioreactor system order, checked before it goes out

An AI sales agent prepares an order for a DASbox Mini Bioreactor System: the cell culture or microbial system, its size, glass or single-use vessels, and the working volume, agitation, gas flow, temperature, feeding lines and sensors the customer needs, with the total working volume it states. Before it goes out, the agent sends it to Predictika, which checks it against a declarative model of the figures Eppendorf publishes in its DASbox brochure and returns a verdict, every reason and the figures it computed.

Scope: DASbox Mini Bioreactor Systems for cell culture and microbial applications (4- to 24-fold, glass or BioBLU 0.3 single-use vessels): published size, vessel, volume, agitation, gas, temperature, feeding and sensor figures. See everything this validator covers

This validator models a DASbox Mini Bioreactor System order, based on the technical data and ordering information in the public Eppendorf DASbox brochure, not just the scenarios shown below.

  • System size and vessels — the system must be a 4-, 8-, 16- or 24-fold DASbox, the bioreactors the customer runs in parallel must fit its positions, and a single-use system needs enough BioBLU 0.3 vessels (sold in sterile four-packs) of the type that belongs to the application: 0.3c and 0.3sc for cell culture, 0.3f for microbial work.
  • Working volume and agitation — the volume per vessel against the range for the vessel (60 to 250 mL for glass; 100 to 250 mL for single-use cell culture; 65 to 250 mL for single-use microbial), the agitation speed against the range for the vessel and impeller (glass up to 2,500 rpm; single-use pitched-blade up to 500 rpm, 8-blade up to 200 rpm and Rushton-type up to 2,000 rpm), and the impeller offered for glass vessels.
  • Gas, temperature and feeding — total gas flow and CO2 flow against the standard ranges of the cell culture system (up to 5 sL/h, CO2 up to 3.5) and the microbial system (up to 25 sL/h, CO2 up to 18), the 10 to 60 degrees Celsius temperature range, and feeding lines (2 standard, 4 with the option).
  • Sensor options and totals — redox measurement (not listed for cell culture; microbial takes redox or level, not both), exhaust gas analysis (microbial only), and the total working volume the quote states, which must equal the computed one.

Any DASbox Mini Bioreactor System order that touches these rules is in scope — not only the scenarios pictured here. Not covered: prices (Eppendorf does not publish list prices for these systems, so this page shows no price build-up), other Eppendorf bioprocess systems such as the BioFlo family, DASware software options, other DASGIP modules, and process performance, which depends on the culture. The brochure gives gas flow in standard liters per hour; the model works in standard milliliters per hour (1 sL is 1,000 standard mL).

The AI sales agentBuilds the system order from the conversation and says it is ready.
Predictika validatorChecks every rule at once and returns the verdict, each finding, and the computed figures.

7 scenarios in 3 groups · each group runs from the agent’s first attempt to the outcome · click one to switch

What the agent proposes

The AI sales agent says

Illustrative agent statement. The validator receives the structured request below.

Proposing agent: AI sales agent (scripted)

Before sending, the agent must call Recorded run

Predictika validator


      

        
        
        
      

      
Waiting for the call.

      
    

  

  
  

    
Where rules-based CPQ gets it wrong
Click to expand
    
Our assessment of what a typical rules-based CPQ (product rules, price rules and validation rules, as in Salesforce CPQ) does with the same cases. It is a judgment about effort and reliability, not a claim that nothing can be built.

    
What the validator caughtTypical rules-based CPQPredictika
Agitation limits that depend on the vessel and its impellerOnly with care
Glass vessels, pitched-blade, 8-blade and Rushton-type single-use vessels each have their own speed range, and the vessel itself depends on the application. A rules-based CPQ needs a lookup per vessel and every brochure update means editing it.		The vessel, its impeller and the speed range are looked up together on every call.
Single-use vessels that belong to one application and must cover the bioreactorsOnly with workarounds
The vessel type depends on the system chosen on another line, and the count is the bioreactors run in parallel against sterile four-packs, which a configurator rarely compares across lines.		System, vessel type, packs and bioreactor count arrive in one request and are compared together.
Sensor options that depend on the application: redox, level and exhaust analysisOnly with care
Some options exist on one system only and two of them exclude each other, so it takes cross-option rules that interact.		Every option is checked against the application with the rest of the order.
Volume, gas and temperature ranges that differ by system and vesselOnly with care
Each range has its own bounds, and the lower and upper limit both apply, so rules multiply with every system and vessel type.		All ranges are looked up from the system and vessel and every bound is checked on each call.
Every problem returned in one answerOnly with workarounds
Rules tend to fire as the order is saved or a step is completed, so a person often fixes one error and meets the next.		All findings come back together, whatever order the choices arrived in.

  

  

    
What this validator catches: findings from its test cases
Click to expand
    
Every rule is checked on every call, in any order the choices arrive; one problem never hides another. Blocking findings stop the quote; approval findings need a person to sign off; everything else is computed.

    
1The quote has a 12-fold system; DASbox systems are offered as 4-, 8-, 16- and 24-fold.
2The customer will run 9 bioreactors in parallel; the quoted system has 8 positions.
3The quote has 4 single-use bioreactors; the customer will run 8.
4The quote has 0 single-use bioreactors; the customer will run 8.
5The selected BioBLU 0.3 vessel is for the other application; cell culture systems use the 0.3c and 0.3sc and microbial systems use the 0.3f.
6The customer needs 1,200 rpm of agitation; this vessel gives 20 to 500 rpm.
7The customer needs 201 rpm of agitation; this vessel gives 20 to 200 rpm.
8The selected impeller is not offered for glass vessels on this system; cell culture glass vessels use marine or 8-blade impellers and microbial glass vessels use Rushton-type impellers.
9The customer needs 59 mL of working volume per vessel; this system and vessel type give 60 to 250 mL.
10The customer needs 99 mL of working volume per vessel; this system and vessel type give 100 to 250 mL.
11The customer needs 251 mL of working volume per vessel; this system and vessel type give 60 to 250 mL.
12The customer needs 2,501 rpm of agitation; this vessel gives 20 to 2,500 rpm.
13The customer needs 19 rpm of agitation; this vessel gives 20 to 2,500 rpm.
14The customer needs 501 rpm of agitation; this vessel gives 20 to 500 rpm.
15The customer needs 2,001 rpm of agitation; this vessel gives 20 to 2,000 rpm.
16The customer needs 5,001 standard mL per hour of gas flow; this system gives 40 to 5000.
17The customer needs 39 standard mL per hour of gas flow; this system gives 40 to 5000.
18The customer needs 3,501 standard mL per hour of CO2; this system gives at most 3500.
19The customer needs 25,001 standard mL per hour of gas flow; this system gives 200 to 25000.
20The customer needs 18,001 standard mL per hour of CO2; this system gives at most 18000.

  

  

    
Scenarios and results
Click to expand
    
The recorded result of the validator for each scenario on this page: the request the AI agent sent, the verdict, and every finding, in the order they appear above.

    
1. Single-use cell culture: Eight-fold single-use cell culture system with a 600 rpm agitation need

    
The agent's request. Please quote a 8-fold DASbox Mini Bioreactor System for cell culture applications with single-use vessels and 2 four-packs of BioBLU 0.3c single-use bioreactors, for a lab that will run 8 bioreactors in parallel at 150 mL of working volume each. The runs need 600 rpm of agitation, 3,000 standard mL per hour of gas flow including 1,500 standard mL per hour of CO2, 37 degrees Celsius and 2 feeding lines per vessel. The quote states 1,200 mL of working volume in total. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • Agitation outside the range. The customer needs 600 rpm of agitation; this vessel gives 20 to 500 rpm.
    • 
    

    
2. Single-use cell culture: The agent resubmits after fixing the errors

    
The agent's request. Please quote a 8-fold DASbox Mini Bioreactor System for cell culture applications with single-use vessels and 2 four-packs of BioBLU 0.3c single-use bioreactors, for a lab that will run 8 bioreactors in parallel at 150 mL of working volume each. The runs need 400 rpm of agitation, 3,000 standard mL per hour of gas flow including 1,500 standard mL per hour of CO2, 37 degrees Celsius and 2 feeding lines per vessel. The quote states 1,200 mL of working volume in total. It is ready to send.

    
Verdict: Allowed.

    
    
      
  • Order summary. 8 bioreactors of 150 mL each, 1,200 mL of working volume in total, on the quoted 8-fold system.
    • 
    

    
3. Microbial glass system: Sixteen-fold microbial glass system with a marine impeller, four feeding lines and both redox and level

    
The agent's request. Please quote a 16-fold DASbox Mini Bioreactor System for microbial applications with glass vessels with a marine impeller, for a lab that will run 12 bioreactors in parallel at 200 mL of working volume each. The runs need 1,500 rpm of agitation, 20,000 standard mL per hour of gas flow including 10,000 standard mL per hour of CO2, 37 degrees Celsius and 4 feeding lines per vessel, and redox measurement and level measurement and exhaust gas analysis. The quote states 2,400 mL of working volume in total. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • Impeller not offered for glass vessels. The selected impeller is not offered for glass vessels on this system; cell culture glass vessels use marine or 8-blade impellers and microbial glass vessels use Rushton-type impellers.
    • 
      
  • Four feeding lines need the option. The customer needs 4 feeding lines per vessel but the quote does not include the 4-feeding-line option; 2 are standard.
    • 
      
  • Redox and level together. The microbial system takes either the redox or the level option, not both.
    • 
    

    
4. Microbial glass system: The agent resubmits after fixing the errors

    
The agent's request. Please quote a 16-fold DASbox Mini Bioreactor System for microbial applications with glass vessels with a Rushton-type impeller, for a lab that will run 12 bioreactors in parallel at 200 mL of working volume each. The runs need 1,500 rpm of agitation, 20,000 standard mL per hour of gas flow including 10,000 standard mL per hour of CO2, 37 degrees Celsius and 4 feeding lines per vessel, with the 4-feeding-line option, and redox measurement and exhaust gas analysis. The quote states 2,400 mL of working volume in total. It is ready to send.

    
Verdict: Allowed.

    
    
      
  • Order summary. 12 bioreactors of 200 mL each, 2,400 mL of working volume in total, on the quoted 16-fold system.
    • 
    

    
5. Single-use vessel for the application: Microbial single-use system with a cell culture vessel and one four-pack

    
The agent's request. Please quote a 8-fold DASbox Mini Bioreactor System for microbial applications with single-use vessels and 1 four-pack of BioBLU 0.3c single-use bioreactors, for a lab that will run 8 bioreactors in parallel at 150 mL of working volume each. The runs need 1,200 rpm of agitation, 15,000 standard mL per hour of gas flow including 5,000 standard mL per hour of CO2, 37 degrees Celsius and 2 feeding lines per vessel. The quote states 1,200 mL of working volume in total. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • Not enough single-use vessels. The quote has 4 single-use bioreactors; the customer will run 8.
    • 
      
  • Single-use vessel is for the other application. The selected BioBLU 0.3 vessel is for the other application; cell culture systems use the 0.3c and 0.3sc and microbial systems use the 0.3f.
    • 
      
  • Agitation outside the range. The customer needs 1,200 rpm of agitation; this vessel gives 20 to 500 rpm.
    • 
    

    
6. Single-use vessel for the application: The agent resubmits after fixing the errors

    
The agent's request. Please quote a 8-fold DASbox Mini Bioreactor System for microbial applications with single-use vessels and 2 four-packs of BioBLU 0.3f single-use bioreactors, for a lab that will run 8 bioreactors in parallel at 150 mL of working volume each. The runs need 1,200 rpm of agitation, 15,000 standard mL per hour of gas flow including 5,000 standard mL per hour of CO2, 37 degrees Celsius and 2 feeding lines per vessel. The quote states 1,200 mL of working volume in total. It is ready to send.

    
Verdict: Allowed.

    
    
      
  • Order summary. 8 bioreactors of 150 mL each, 1,200 mL of working volume in total, on the quoted 8-fold system.
    • 
    

    
7. Cell culture glass system: Four-fold cell culture system with six bioreactors, redox, exhaust analysis and a wrong total

    
The agent's request. Please quote a 4-fold DASbox Mini Bioreactor System for cell culture applications with glass vessels with a marine impeller, for a lab that will run 6 bioreactors in parallel at 200 mL of working volume each. The runs need 400 rpm of agitation, 2,000 standard mL per hour of gas flow including 800 standard mL per hour of CO2, 37 degrees Celsius and 2 feeding lines per vessel, and redox measurement and exhaust gas analysis. The quote states 1,000 mL of working volume in total. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • More bioreactors than the system holds. The customer will run 6 bioreactors in parallel; the quoted system has 4 positions.
    • 
      
  • Redox measurement not on cell culture system. Redox (ORP) measurement is not listed for the cell culture system.
    • 
      
  • Exhaust analysis only on microbial system. Exhaust gas analysis (GA4) is listed only for the microbial system.
    • 
      
  • Stated total volume does not match. The quote states 1,000 mL of working volume in total; the bioreactors give 1200.
    • 
    

  

  

    
Narration transcript
Click to expand
    
The text of the narrated walkthrough, step by step.

    
    
      
  1. Welcome to a demo of Predictika's Validator. An AI sales agent prepares an order for a DASbox Mini Bioreactor System: the cell culture or microbial system, its size, glass or single-use vessels, and more. Before it goes out, the agent sends it to Predictika, which checks it against a declarative model of the figures Eppendorf publishes in its DASbox brochure and returns a verdict, every reason and the figures it computed.
    2. 
      
  2. I will start with the first request by the agent.
    3. 
      
  3. The agent says. Please quote a 8-fold DASbox Mini Bioreactor System for cell culture applications. The runs need 600 rpm of agitation, 3,000 standard mL per hour of gas flow including 1,500 standard mL per hour of CO2. The rest of the request is on screen. The quote states 1,200 mL of working volume in total.
    4. 
      
  4. Before it goes out, the agent calls the validator.
    5. 
      
  5. Blocked, with one finding, all in one answer. The customer needs 600 rpm of agitation; this vessel gives 20 to 500 rpm.
    6. 
      
  6. The validator returned one blocking finding in one answer. A check that reads one field at a time would pass most of this; it shows only when the choices in the quote are checked against each other.
    7. 
      
  7. What this shows: for agitation limits that depend on the vessel and its impeller, a rules-based CPQ is error-prone for this.
    8. 
      
  8. Now the agent resubmits.
    9. 
      
  9. The corrected request. It changes the agitation needed to 400 rpm.
    10. 
      
  10. It calls the validator again.
    11. 
      
  11. This time the validator clears it.
    12. 
      
  12. The earlier finding is cleared, and the validator's figure matches the quote.
    13. 
      
  13. One problem caught in one call, fixed in one pass, and a clean quote. That is the value of Predictika's validator: catching mistakes before they reach the customer. Now you can try the other scenarios yourself.
    14.