Predictika · Life sciences reagents and testing kits

An AI sales agent's mRNA reagent and host cell protein test kit order, checked before it goes out

An AI sales agent prepares an order for the Maravai LifeSciences businesses: TriLink BioTechnologies CleanCap capping reagents, N1-methylpseudouridine and other nucleotides, in vitro transcription enzymes, reporter and custom mRNA, in research or GMP grade, and Cygnus Technologies host cell protein ELISA kits, for a research, preclinical or clinical program. Before the quote goes out, the agent sends it to Predictika, which checks it against a declarative model of the public TriLink and Cygnus product pages and returns a verdict, every reason, a line-item quote and the figures it computed.

Scope: TriLink CleanCap capping reagents (AG, AU, AG 3′ OMe, M6), N1-methylpseudouridine and other NTPs, IVT enzymes, FLuc and custom mRNA in research or GMP grade, and Cygnus CHO and Vero host cell protein ELISA kits, for any program size. See everything this validator covers

This validator models orders for the TriLink BioTechnologies and Cygnus Technologies products below, based on their public product pages, inserts and flyers. It handles any order for these products, not just the scenarios shown. Where a rule is a published fact it says so; reaction counts per pack, plate capacity, custom batch limits and prices that are only assumed or typical are labeled that way.

  • TriLink capping reagents — CleanCap Reagent AG (N-7113, GMP FN-7113), AU (N-7114), AG (3′ OMe) (N-7413) and M6 (N-7453), each tied to the 5′ start of the mRNA template, and CleanCap AG for self-amplifying RNA.
  • Nucleotides and enzymes — N1-methylpseudouridine-5′-triphosphate (N-1081, GMP FN-1081), ATP, CTP and GTP (N-1501 to N-1503), T7 RNA polymerase (E-0053), RNase inhibitor (E-0075) and pyrophosphatase (E-0051) in 25- and 250-reaction packs, checked against the number of 100 µL IVT reactions planned.
  • mRNA — CleanCap M6 FLuc mRNA (L-8102, stored at or below −40 °C) and custom mRNA or self-amplifying RNA manufacturing, research or GMP grade under a quality agreement.
  • Cygnus host cell protein testing — CHO HCP ELISA Kit 3G (F550-1), the Robotics Kit for the Hamilton Microlab STAR (F550-1-4), the depleted F550-4, Standards Set A-F (F553-1), Vero Cell 2G (F975) and 3G (F1095) HCP ELISA kits and Sample Diluent Buffer (I028), stored at 2 to 8 °C.
  • What the validator checks — grade against program stage, GMP availability and the quality agreement, cap against template start, reactions covered by every reagent, storage at the receiving site, frozen and refrigerated products in one shipment, host cell line against kit, plates and diluent for the samples, custom batch minimums and lengths, discount and the stated total. Prices on inquiry, a large order value, a discount above 8 percent and kits being resupplied go to a person.
  • Assumed or typical (not published) — every price except the N-7113 and N-1081 list prices, 25 reactions per 10 µmol of cap and 20 per 10 µmol of NTP, 40 samples per plate and one plate per single kit, GMP grade only for CleanCap AG and N1-methylpseudouridine, the start rule for AG (3′ OMe) and M6, custom mRNA minimums, lengths and rates, one diluent bottle per 5 plates, the shipping split, the 15 percent discount limit and the approval thresholds.

Any order for these products that touches these rules is in scope — not only the scenarios pictured here. Not covered: other TriLink products (oligonucleotides, plasmids, other mRNA catalog items), other Cygnus kits and antibodies, Hongene, Maravai Biologics Safety Testing services, freight and taxes. The customers are fictional.

The AI sales agentBuilds the order from the conversation with the customer and says it is ready.
Predictika validatorChecks every rule at once and returns the verdict, each finding, and the computed figures.

6 scenarios in 3 groups · each group runs from the agent’s first attempt to the outcome · click one to switch

What the agent proposes

The AI sales agent says

Illustrative agent statement. The validator receives the structured request below.

Proposing agent: AI sales agent (scripted)

Before sending, the agent must call Recorded run

Predictika validator


      

        
        
        
      

      
Waiting for the call.

      
    

  

  
  

    
Where rules-based CPQ gets it wrong
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Our assessment of what a typical rules-based CPQ (product rules, price rules and validation rules, as in Salesforce CPQ) does with the same cases. It is a judgment about effort and reliability, not a claim that nothing can be built.

    
What the validator caughtTypical rules-based CPQPredictika
Research vs GMP grade that follows the program stageOnly with workarounds
A clinical program needs GMP-grade cap and N1-methylpseudouridine, with a signed quality agreement; a research-use line on a clinical order is a hand-kept rule between a program field and a grade on each product line.		Grade, program and quality agreement are checked together on every line.
A capping reagent that must match the template startOnly with workarounds
CleanCap AU is for AU starts and AG, AG (3′ OMe) and M6 for AG starts. The limit sits on a template attribute that quote forms rarely ask for, so it becomes a table of compatibility pairs kept by hand.		The cap is checked against the template start it will be used with.
Every reagent covering the same number of reactionsOnly with workarounds
Cap, nucleotides and three enzymes come in 10 and 100 µmol and 25- and 250-reaction packs. Whether the packs ordered cover 2,000 reactions is arithmetic across a dozen SKUs, which most quote setups do not carry.		Reactions covered are computed for each reagent and compared with the plan.
Frozen and 2 to 8 °C products in one shipmentNot without custom objects
Rules-based CPQ has no shipment temperature class; it needs a custom field on every product and a rule that reads the whole cart.		Storage classes are read from the lines and checked against the shipping choice.
Facts about the customer's laboratoryOnly with workarounds
A robotics kit needs a Hamilton Microlab STAR, FLuc mRNA needs a freezer at −40 °C or colder, and an HCP kit must match the host cell line. The quote form does not hold these answers.		The laboratory facts are inputs and are checked against the products.
Plates and diluent for the samplesOnly with care
A single kit and a four-plate robotics kit supply different plate counts, and diluent bottles cover a set number of plates. Doable with summary variables, but fragile.		Samples, plates and diluent are compared in one pass.
Guidance that needs a person, such as prices given on inquiryOnly with workarounds
GMP prices are not on a list. Quote tools tend to ignore the case or block the quote outright.		The quote is not blocked; it goes to a person with the reason listed.
A stated total that has to agree with the lines and the discountOnly with workarounds
The total typed on the quote can drift from dozens of lines without anyone noticing.		The total is computed from the lines and compared with the total the quote states.
Every problem returned in one answerOnly with workarounds
Rules tend to fire as the quote is saved or a step is completed, so a person often fixes one error and meets the next.		All findings come back together, whatever order the choices arrived in.

  

  

    
What this validator catches: findings from its test cases
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Every rule is checked on every call, in any order the choices arrive; one problem never hides another. Blocking findings stop the quote; approval findings need a person to sign off; everything else is computed.

    
1The CleanCap reagent does not match the 5′ start of the mRNA template; CleanCap AG, AG (3′ OMe) and M6 are for templates that start with AG, and CleanCap AU is for templates that start with AU.
2The order plans 100 IVT reactions and includes no capping reagent.
3The order includes items that TriLink prices on inquiry (GMP-grade material, the 100 µmol CleanCap AG pack or a GMP mRNA batch); sales operations confirms the quoted prices.
4The program is clinical, which needs GMP-grade capping reagent, and the capping reagent on the order is research use only.
5The program is clinical, which needs GMP-grade N1-methylpseudouridine-5-triphosphate, and the N1-methylpseudouridine-5-triphosphate on the order is research use only.
6The program is not clinical and the order includes GMP-grade material; a person confirms that GMP grade is intended.
7GMP grade is listed for CleanCap Reagent AG only (FN-7113); the selected CleanCap reagent has no GMP grade.
8The order includes GMP-grade material, which is made under a GMP quality agreement, and no signed quality agreement is on file.
9The order plans 100 IVT reactions of 100 microliters, and the capping reagent ordered covers 75.
10The order plans 100 IVT reactions, and the N1-methylpseudouridine-5-triphosphate ordered covers 80.
11The order plans 100 IVT reactions, and the ATP, CTP and GTP ordered cover 80.
12The order plans 100 IVT reactions, and the T7 RNA polymerase, RNase inhibitor and pyrophosphatase ordered cover 75, 100 and 100.
13CleanCap M6 FLuc mRNA (L-8102) is stored at or below −40 °C, and the receiving site has a −20 °C freezer.
14The order mixes frozen TriLink products with Cygnus kits stored at 2 to 8 °C and is set to ship as a single shipment; the two temperature classes ship separately.
15The order includes 1 of catalog number F550-4, which Cygnus lists as depleted and replaced by the CHO HCP ELISA 3G Robotics Kit (F550-1-4).
16The order includes a robotics kit, which is made for the Hamilton Microlab STAR system, and the laboratory has no Hamilton Microlab STAR.
17The order includes CHO host cell protein products (the CHO HCP ELISA kits or standards) and the process host cell is not CHO.
18The order includes 1 Vero Cell 2G HCP ELISA Kits (F975); Cygnus says F975 is being resupplied and the Vero Cell 3G kit (F1095) is available, so sales operations confirms availability.
19The order includes Vero cell host cell protein ELISA kits and the process host cell is not Vero.
20The plan tests 41 samples in duplicate, and the kits ordered supply 1 plate, which test up to 40 samples at 40 samples per plate.
21The kits supply 1 plate and the order includes 0 bottles of Sample Diluent Buffer (I028); one bottle covers 5 plates.
22The custom mRNA batch is 1 mg, and the minimum batch for this program is 2 mg.
23The custom RNA is 5,001 nucleotides long, and this validator allows up to 5,000 nucleotides for this kind of RNA.
24A self-amplifying RNA batch needs CleanCap Reagent AG, which is designed for self-amplifying RNA, and the order has a different capping reagent.
25The order includes a custom mRNA batch and no capping reagent is selected for it.
26The order includes no TriLink or Cygnus product, so there is nothing to price.
27The quote states a total of $10,371, but the products, services and discount give $10,372.
28A discount of $830 on a list price of $10,372 is more than 8 percent and needs a manager to approve it.
29A discount of $1,556 on a list price of $10,372 is more than the 15 percent limit.
30The net order value of $631,260 is above $250,000; an account director approves it.

  

  

    
Scenarios and results
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The recorded result of the validator for each scenario on this page: the request the AI agent sent, the verdict, and every finding, in the order they appear above.

    
1. Clinical-stage biotech, mRNA program and CHO antibody: A large order for a clinical mRNA program and CHO antibody release testing

    
The agent's request. Please quote an order for a clinical program with 2,000 IVT reactions of 100 µL on a template that starts with AG, and the process uses CHO cells. Capping: 6 packs of 100 µmol of CleanCap Reagent AU, research use. Nucleotides: 10 packs of 100 µmol of N1-methylpseudouridine-5′-triphosphate, research use; 10 packs of 100 µmol each of ATP, CTP and GTP. Enzymes: 8 packs of 250 reactions of T7 RNA polymerase; 8 packs of 250 reactions of RNase inhibitor; 6 packs of 250 reactions of pyrophosphatase. Host cell protein testing: 2 CHO HCP ELISA 3G Robotics Kits (F550-4), 1 CHO HCP Standards Set A-F, 3G (F553-1) and 2 bottles of Sample Diluent Buffer (I028), for 300 samples in duplicate. It ships as one shipment. The discount is $12,000. The quote states $155,920. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • CleanCap reagent does not match the template start. The CleanCap reagent does not match the 5′ start of the mRNA template; CleanCap AG, AG (3′ OMe) and M6 are for templates that start with AG, and CleanCap AU is for templates that start with AU.
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  • Research-use capping reagent in a clinical program. The program is clinical, which needs GMP-grade capping reagent, and the capping reagent on the order is research use only.
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  • Research-use N1-methylpseudo-UTP in a clinical program. The program is clinical, which needs GMP-grade N1-methylpseudouridine-5-triphosphate, and the N1-methylpseudouridine-5-triphosphate on the order is research use only.
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  • Capping reagent covers too few reactions. The order plans 2,000 IVT reactions of 100 microliters, and the capping reagent ordered covers 1,500.
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  • IVT enzymes cover too few reactions. The order plans 2,000 IVT reactions, and the T7 RNA polymerase, RNase inhibitor and pyrophosphatase ordered cover 2000, 2,000 and 1,500.
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  • Frozen and refrigerated products in one shipment. The order mixes frozen TriLink products with Cygnus kits stored at 2 to 8 °C and is set to ship as a single shipment; the two temperature classes ship separately.
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  • F550-4 is depleted. The order includes 2 of catalog number F550-4, which Cygnus lists as depleted and replaced by the CHO HCP ELISA 3G Robotics Kit (F550-1-4).
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  • Robotics kit without a Hamilton Microlab STAR. The order includes a robotics kit, which is made for the Hamilton Microlab STAR system, and the laboratory has no Hamilton Microlab STAR.
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  • Stated total differs from the computed total. The quote states a total of $155,920, but the products, services and discount give $159,540.
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2. Clinical-stage biotech, mRNA program and CHO antibody: The agent resubmits after fixing the errors

    
The agent's request. Please quote an order for a clinical program with 2,000 IVT reactions of 100 µL on a template that starts with AG, and the process uses CHO cells. Capping: 8 packs of 100 µmol of CleanCap Reagent AG, GMP grade. Nucleotides: 10 packs of 100 µmol of N1-methylpseudouridine-5′-triphosphate, GMP grade; 10 packs of 100 µmol each of ATP, CTP and GTP. Enzymes: 8 packs of 250 reactions of T7 RNA polymerase; 8 packs of 250 reactions of RNase inhibitor; 8 packs of 250 reactions of pyrophosphatase. Host cell protein testing: 8 CHO HCP ELISA Kits, 3G (F550-1), 1 CHO HCP Standards Set A-F, 3G (F553-1) and 2 bottles of Sample Diluent Buffer (I028), for 300 samples in duplicate. It ships as separate shipments for frozen and refrigerated products. The discount is $12,000. The quote states $452,380. It is ready to send.

    
Verdict: Needs approval.

    
    
      
  • Prices given on inquiry. The order includes items that TriLink prices on inquiry (GMP-grade material, the 100 µmol CleanCap AG pack or a GMP mRNA batch); sales operations confirms the quoted prices.
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  • Order value above $250,000. The net order value of $452,380 is above $250,000; an account director approves it.
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  • Order summary. List price $464,380, discount $12,000, net $452,380 (illustrative prices, not published prices).
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3. Preclinical research group: An order for IVT reagents, a FLuc control and host cell protein kits

    
The agent's request. Please quote an order for a preclinical program with 300 IVT reactions of 100 µL on a template that starts with AG, and the process uses CHO cells. Capping: 12 packs of 10 µmol of CleanCap Reagent AG (3′ OMe), research use. Nucleotides: 10 packs of 10 µmol of N1-methylpseudouridine-5′-triphosphate, research use; 15 packs of 10 µmol each of ATP, CTP and GTP. Enzymes: 12 packs of 25 reactions of T7 RNA polymerase; 12 packs of 25 reactions of RNase inhibitor; 12 packs of 25 reactions of pyrophosphatase. Control: 1 vial of 1 mg of CleanCap M6 FLuc mRNA. Host cell protein testing: 2 Vero Cell 3G HCP ELISA Kits (F1095) and 1 bottle of Sample Diluent Buffer (I028), for 120 samples in duplicate. It ships as separate shipments for frozen and refrigerated products. The discount is $6,700. The quote states $31,075. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • N1-methylpseudo-UTP covers too few reactions. The order plans 300 IVT reactions, and the N1-methylpseudouridine-5-triphosphate ordered covers 200.
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  • FLuc mRNA needs a freezer at minus 40 or colder. CleanCap M6 FLuc mRNA (L-8102) is stored at or below −40 °C, and the receiving site has a −20 °C freezer.
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  • Vero kits for a process that is not Vero. The order includes Vero cell host cell protein ELISA kits and the process host cell is not Vero.
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  • Too few plates for the samples. The plan tests 120 samples in duplicate, and the kits ordered supply 2 plates, which test up to 80 samples at 40 samples per plate.
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  • Discount above 15 percent. A discount of $6,700 on a list price of $38,925 is more than the 15 percent limit.
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  • Stated total differs from the computed total. The quote states a total of $31,075, but the products, services and discount give $32,225.
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  • Discount above 8 percent. A discount of $6,700 on a list price of $38,925 is more than 8 percent and needs a manager to approve it.
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4. Preclinical research group: The agent resubmits after fixing the errors

    
The agent's request. Please quote an order for a preclinical program with 300 IVT reactions of 100 µL on a template that starts with AG, and the process uses CHO cells. Capping: 12 packs of 10 µmol of CleanCap Reagent AG (3′ OMe), research use. Nucleotides: 15 packs of 10 µmol of N1-methylpseudouridine-5′-triphosphate, research use; 15 packs of 10 µmol each of ATP, CTP and GTP. Enzymes: 12 packs of 25 reactions of T7 RNA polymerase; 12 packs of 25 reactions of RNase inhibitor; 12 packs of 25 reactions of pyrophosphatase. Host cell protein testing: 3 CHO HCP ELISA Kits, 3G (F550-1) and 1 bottle of Sample Diluent Buffer (I028), for 120 samples in duplicate. It ships as separate shipments for frozen and refrigerated products. The discount is $2,500. The quote states $40,250. It is ready to send.

    
Verdict: Allowed.

    
    
      
  • Order summary. List price $42,750, discount $2,500, net $40,250 (illustrative prices, not published prices).
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5. Vaccine developer, self-amplifying RNA: An order for a self-amplifying RNA batch, IVT reagents and Vero process testing

    
The agent's request. Please quote an order for a preclinical program with 200 IVT reactions of 100 µL on a template that starts with AG, and the process uses Vero cells. Capping: 8 packs of 10 µmol of CleanCap Reagent AU, research use. Nucleotides: 10 packs of 10 µmol of N1-methylpseudouridine-5′-triphosphate, research use; 10 packs of 10 µmol each of ATP, CTP and GTP. Enzymes: 8 packs of 25 reactions of T7 RNA polymerase; 8 packs of 25 reactions of RNase inhibitor; 8 packs of 25 reactions of pyrophosphatase. Custom self-amplifying RNA: a batch of 1 mg, 13,500 nucleotides long. Host cell protein testing: 1 CHO HCP ELISA Kit, 3G (F550-1), 2 Vero Cell 3G HCP ELISA Kits (F1095) and 1 bottle of Sample Diluent Buffer (I028), for 70 samples in duplicate. It ships as separate shipments for frozen and refrigerated products. The discount is $1,000. The quote states $36,554. It is ready to send.

    
Verdict: Blocked.

    
    
      
  • CleanCap reagent does not match the template start. The CleanCap reagent does not match the 5′ start of the mRNA template; CleanCap AG, AG (3′ OMe) and M6 are for templates that start with AG, and CleanCap AU is for templates that start with AU.
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  • CHO products for a process that is not CHO. The order includes CHO host cell protein products (the CHO HCP ELISA kits or standards) and the process host cell is not CHO.
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  • Self-amplifying RNA needs CleanCap AG. A self-amplifying RNA batch needs CleanCap Reagent AG, which is designed for self-amplifying RNA, and the order has a different capping reagent.
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  • Custom mRNA batch below the minimum. The custom mRNA batch is 1 mg, and the minimum batch for this program is 2 mg.
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  • Custom RNA longer than the limit. The custom RNA is 13,500 nucleotides long, and this validator allows up to 12,000 nucleotides for this kind of RNA.
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  • Stated total differs from the computed total. The quote states a total of $36,554, but the products, services and discount give $32,054.
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6. Vaccine developer, self-amplifying RNA: The agent resubmits after fixing the errors

    
The agent's request. Please quote an order for a preclinical program with 200 IVT reactions of 100 µL on a template that starts with AG, and the process uses Vero cells. Capping: 8 packs of 10 µmol of CleanCap Reagent AG, research use. Nucleotides: 10 packs of 10 µmol of N1-methylpseudouridine-5′-triphosphate, research use; 10 packs of 10 µmol each of ATP, CTP and GTP. Enzymes: 8 packs of 25 reactions of T7 RNA polymerase; 8 packs of 25 reactions of RNase inhibitor; 8 packs of 25 reactions of pyrophosphatase. Custom self-amplifying RNA: a batch of 4 mg, 11,500 nucleotides long. Host cell protein testing: 3 Vero Cell 3G HCP ELISA Kits (F1095) and 1 bottle of Sample Diluent Buffer (I028), for 70 samples in duplicate. It ships as separate shipments for frozen and refrigerated products. The discount is $1,000. The quote states $41,254. It is ready to send.

    
Verdict: Allowed.

    
    
      
  • Order summary. List price $42,254, discount $1,000, net $41,254 (illustrative prices, not published prices).
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Narration transcript
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The text of the narrated walkthrough, step by step.

    
    
      
  1. Welcome to a demo of Predictika's Validator. An AI sales agent prepares an order for the Maravai LifeSciences businesses: TriLink BioTechnologies CleanCap capping reagents, N1-methylpseudouridine and other nucleotides, in vitro transcription enzymes, and more. Before the quote goes out, the agent sends it to Predictika, which checks it against a declarative model of the public TriLink and Cygnus product pages and returns a verdict, every reason, a line-item quote and the figures it computed.
    2. 
      
  2. I will start with the first request by the agent.
    3. 
      
  3. The agent says. Please quote an order for a clinical program with 2,000 IVT reactions of 100 µL on a template that starts with AG, and the process uses CHO cells. Capping: 6 packs of 100 µmol of CleanCap Reagent AU, research use. The rest of the request is on screen. The quote states $155,920.
    4. 
      
  4. Before it goes out, the agent calls the validator.
    5. 
      
  5. Blocked, with nine findings, all in one answer. The CleanCap reagent does not match the 5′ start of the mRNA template; CleanCap AG, AG (3′ OMe) and M6 are for templates that start with AG, and CleanCap AU is for templates that start with AU. The program is clinical, which needs GMP-grade capping reagent, and the capping reagent on the order is research use only. The program is clinical, which needs GMP-grade N1-methylpseudouridine-5-triphosphate, and the N1-methylpseudouridine-5-triphosphate on the order is research use only. The other six are on screen.
    6. 
      
  6. The validator returned nine blocking findings in one answer. A check that reads one field at a time would pass most of this; it shows only when the choices in the quote are checked against each other and against the stated total.
    7. 
      
  7. What this shows: for a capping reagent that must match the template start, a rules-based CPQ can do this only with workarounds.
    8. 
      
  8. Now the agent resubmits.
    9. 
      
  9. The corrected request. It changes the capping reagent to CleanCap Reagent AG, GMP grade, the cap packs of 100 µmol to 8, the N1-methylpseudo-UTP grade to GMP grade, the pyrophosphatase packs of 250 reactions to 8 and the stated total to $452,380. Two more changes are on screen. It removes the F550-4 Robotics Kit.
    10. 
      
  10. It calls the validator again.
    11. 
      
  11. Not blocked, but held for a person.
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  12. The validator also returns the line-item quote. It starts with CleanCap Reagent AG and N1-Methylpseudouridine-5-Triphosphate; the other nine lines are on screen.
    13. 
      
  13. The nine blocking findings are cleared; two approvals remain for a person: prices given on inquiry and order value above $250,000.
    14. 
      
  14. Nine problems caught in one call, fixed in one pass, and the corrected quote now goes to a person for approval. That is the value of Predictika's validator: catching mistakes before they reach the customer. Now you can try the other scenarios yourself.
    15.